Session for Polly Peptide, March 2, 2001 (T1) Vinay Chaudri Sample Session: Create a concept by starting from Action Give it a name RNA-Transcription (RNAT) Add concept DNA Add concept mRNA Add concept RNA polymerase Connect RNAT to RNA polymerase by an instrument link Connect RNAT to DNA by an object link Connect RNAT and mRNA by object link Abort Create a concept by starting from Action Give it a name RNA-Transcription (RNAT) Add concept DNA Add concept mRNA Add concept RNA polymerase Connect RNAT to RNA polymerase by an instrument link Connect RNAT to DNA by an object link Add concept Make-Contact Search for Slide Search for and add Move-to Search for Promoter and add Search for Terminator and add Search for Terminator and add Connect Make-contact with RNAT by a subevent link Connect Move-to with RNAT by a subevent link Merge promoter and DNA Merge promoter region with object of contact Merge object of contact with carboxyl terminal end of Merge "thing moved" of Move-to to Terminator Region Make transcribe a subevent of RNAT Abort Create a concept by starting from Action Give it a name RNA-Transcription (RNAT) Add concept Eucaroyatic-DNA Abort Create a concept by starting from Action Give it a name RNA-Transcription (RNAT) Add concept DNA Search DNA Open DNA Select Bacterial DNA Search and Add RNA-Polymerase Connect RNA-T with DNA by object link Connect RNA-T with Polymerase with instrument link Search for Attach Add Make-contact from history list Connect Make-contact and RNA-polymerase by an object link Connect Make-contact and DNA by the object link Connect Make-Contact to RNAT by subevent link Search for move. Add move step. Connect RNAT to move by subevent. Connect object of Move to Promoter of DNA Search for attach. Add attach. Connect RNAT and attach by subevent link. Connect object to RNA-polymerase Connect object to RNA-polymerase Search for transcribe Add Transcribe step Make Transcribe a substep of RNAT Search for mRNA Add mRNA Make it object of transcribe Make RNA polymerase an instrument of transcribe Search for release Add release step Make Release a subevent of RNAT Make mRNA an object of Release (this did not work due to buggy software) Search for detach Add Brea-Contact Make it a subevent of RNAT Make terminator an object of things breaking contact Save Ask question What is RNA Transcription Two Break-Contact subevents show up, only one was put in Click on the instrucment of RNA Transcription Click on the object of RNA Transcription. Its labeled as O2. RNA polymerase shows up as an object, but it was input as an instrument Ask how many instruments, answer =1, correct. Ask how many object, answer = 1, correct. Ask how many subevents, answer = 7, correct. Ask what role does DNA play - no answer. Ask what role does RNA polumerase play? - no answer. What happens to location of RNA polymerase? -shows 7 locations, but not very informative What happens to location of DNA? -shows 7 locations, but not very informative