Abstract

Current biomarkers for myocardial infarction (MI) diagnosis are typically late markers released upon cell death, incapable of distinguishing between ischemic and reperfusion injury and can be symptoms of other pathologies. Circulating microRNAs (miRNAs) have recently been proposed as alternative biomarkers for MI diagnosis; however, detecting the changes in the human cardiac miRNA profile during MI is extremely difficult. Here, to study the changes in miRNA levels during acute MI, a heart‐on‐chip model with a cardiac channel, containing human induced pluripotent stem cell (hiPSC)‐derived cardiomyocytes in human heart decellularized matrix and collagen, and a vascular channel, containing hiPSC‐derived endothelial cells, is developed. This model is exposed to anoxia followed by normoxia to mimic ischemia and reperfusion, respectively. Using a highly sensitive miRNA biosensor that the authors …

Date

2022

Authors

Bradley W Ellis, George Ronan, Xiang Ren, Gokhan Bahcecioglu, Satyajyoti Senapati, David Anderson, Eileen Handberg, Keith L March, Hsueh‐Chia Chang, Pinar Zorlutuna

Journal

Small

Volume

18

Issue

28

Pages

2201330