Abstract

Studies of the mechanical characteristics of single cancer cells have shown that non-metastatic and metastatic cells have significant differences in stiffness. We used a microfluidic multi-constriction channel device to differentiate cancer cells and normal cells based upon differences in their mechanical properties.
We fabricated the multi-constriction channels on a silicon wafer with SU-8 photolithography and polydimethylsiloxane (PDMS) soft-lithography, followed by PDMS and glass bonding after plasma treatment. The multi-constriction microfluidic channel contains five constriction channels each 50 μm in length separated by relaxation sections of 50 μm between every constriction channel. We used MDA-MB-231 as the cancer cell line and MCF-10A as the normal cell line, and utilized a smart phone to record video via a microscope. Our measurement by smart phone slow-motion video through microscope reliably …

Date

2017

Authors

Xiang Ren, Parham Ghassemi, Hesam Babahosseini, Jeannine S Strobl, Masoud Agah

Journal

Cancer Research

Volume

77

Issue

13_Supplement

Pages

3924-3924

Publisher

The American Association for Cancer Research