Abstract

Despite its ultrasensitive detection capability, surface-enhanced Raman spectroscopy (SERS) faces challenges as a quantitative biochemical analysis due to the significant dependence of local field enhancement on nanoscale geometric variations. Significant efforts have been devoted to develop SERS calibration methods by introducing Raman tags as internal standards. Raman tags undergo similar SERS enhancement, and ratiometric signals for target analytes can be generated with reduced SERS enhancement variations. However, using Raman tags still faces challenges for label-free applications, including spatial competition between the analyte and tags in hotspots, spectral interference, limited long-term stability due to laser-induced photo-degradation. We demonstrate that electronic Raman scattering (ERS) signals from metallic nanostructures at hotspots can serve as the internal calibration standard to …

Date

2022

Authors

Wonil Nam, Yuming Zhao, Xiang Ren, Masoud Agah, Inyoung Kim, Wei Zhou

Conference

Biomedical Spectroscopy, Microscopy, and Imaging II

Volume

12144

Pages

19-23

Publisher

SPIE