Abstract

Defining the structural and functional changes in the nervous system underlying learning and memory represents a major challenge for modern neuroscience. Although changes in neuronal activity following memory formation have been studied [B. F. Grewe et al., Nature 543, 670–675 (2017); M. T. Rogan, U. V. Stäubli, J. E. LeDoux, Nature 390, 604–607 (1997)], the underlying structural changes at the synapse level remain poorly understood. Here, we capture synaptic changes in the midlarval zebrafish brain that occur during associative memory formation by imaging excitatory synapses labeled with recombinant probes using selective plane illumination microscopy. Imaging the same subjects before and after classical conditioning at single-synapse resolution provides an unbiased mapping of synaptic changes accompanying memory formation. In control animals and animals that failed to learn the task, there …

Date

2022

Authors

William P Dempsey, Zhuowei Du, Anna Nadtochiy, Colton D Smith, Karl Czajkowski, Andrey Andreev, Drew N Robson, Jennifer M Li, Serina Applebaum, Thai V Truong, Carl Kesselman, Scott E Fraser, Don B Arnold

Journal

Proceedings of the National Academy of Sciences

Volume

119

Issue

3

Pages

e2107661119

Publisher

National Academy of Sciences