Abstract

In this study, an ion depleted zone created by an ion-selective membrane was used to impose a high and uniform constant extracellular potential over an entire ∼1000 cell rat cardiomyocyte (rCM) colony on-a-chip to trigger synchronized voltage-gated ion channel activities while preserving cell viability, thus extending single-cell voltage-clamp ion channel studies to an entire normalized colony. Image analysis indicated that rCM beating was strengthened and accelerated (by a factor of ∼2) within minutes of ion depletion and the duration of contraction and relaxation phases was significantly reduced. After the initial synchronization, the entire colony responds collectively to external potential changes such that beating over the entire colony can be activated or deactivated within 0.1 s. These newly observed collective dynamic responses, due to simultaneous ion channel activation/deactivation by a uniform constant …

Date

2020

Authors

Vivek Yadav, Nicholas Chong, Bradley Ellis, Xiang Ren, Satyajyoti Senapati, Hsueh-Chia Chang, Pinar Zorlutuna

Journal

Lab on a Chip

Volume

20

Issue

22

Pages

4273-4284

Publisher

Royal Society of Chemistry